Preparation and characterization of SARS in-house reference antiserum
Identifieur interne : 004717 ( Main/Exploration ); précédent : 004716; suivant : 004718Preparation and characterization of SARS in-house reference antiserum
Auteurs : Jiansan Zhang [République populaire de Chine] ; Yuxuan Liu [République populaire de Chine] ; Liangxiang Hu [République populaire de Chine] ; Qiang Gao [République populaire de Chine] ; Zhenshan Zhang [République populaire de Chine] ; Xiaomei Zhang [République populaire de Chine] ; Jiangting Chen [République populaire de Chine] ; Xuejie Gong [République populaire de Chine] ; Lifei Song [République populaire de Chine] ; Yufen Liu [République populaire de Chine] ; Jing Li [République populaire de Chine] ; Shufen Li [République populaire de Chine] ; Jinfeng Huang [République populaire de Chine] ; Ye Ning [République populaire de Chine] ; Hong Gao [République populaire de Chine] ; Chuan Qin [République populaire de Chine] ; Xiaoping Dong [République populaire de Chine] ; Jianguo Wei [République populaire de Chine] ; Guanmu Dong [République populaire de Chine] ; Weidong Yin [République populaire de Chine]Source :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Cellules Vero, Convalescence, Humains, Normes de référence, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (sang), Tests de neutralisation, Tests sérologiques (normes), Virus du SRAS (immunologie), Virus du SRAS (physiologie).
- MESH :
- immunologie : Syndrome respiratoire aigu sévère, Virus du SRAS.
- normes : Tests sérologiques.
- physiologie : Virus du SRAS.
- sang : Anticorps antiviraux, Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Convalescence, Humains, Normes de référence, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Chlorocebus aethiops, Convalescence, Humans, Neutralization Tests, Reference Standards, SARS Virus (immunology), SARS Virus (physiology), Serologic Tests (standards), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (immunology), Vero Cells.
- MESH :
- chemical , blood : Antibodies, Viral.
- blood : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- physiology : SARS Virus.
- standards : Serologic Tests.
- Animals, Chlorocebus aethiops, Convalescence, Humans, Neutralization Tests, Reference Standards, Vero Cells.
Abstract
A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum.
Url:
DOI: 10.1016/j.vaccine.2004.12.032
PubMed: 16269206
PubMed Central: 7115399
Affiliations:
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Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Yuxuan" sort="Liu, Yuxuan" uniqKey="Liu Y" first="Yuxuan" last="Liu">Yuxuan Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hu, Liangxiang" sort="Hu, Liangxiang" uniqKey="Hu L" first="Liangxiang" last="Hu">Liangxiang Hu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Gao, Qiang" sort="Gao, Qiang" uniqKey="Gao Q" first="Qiang" last="Gao">Qiang Gao</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Zhenshan" sort="Zhang, Zhenshan" uniqKey="Zhang Z" first="Zhenshan" last="Zhang">Zhenshan Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. 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Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Shufen" sort="Li, Shufen" uniqKey="Li S" first="Shufen" last="Li">Shufen Li</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Huang, Jinfeng" sort="Huang, Jinfeng" uniqKey="Huang J" first="Jinfeng" last="Huang">Jinfeng Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ning, Ye" sort="Ning, Ye" uniqKey="Ning Y" first="Ye" last="Ning">Ye Ning</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Gao, Hong" sort="Gao, Hong" uniqKey="Gao H" first="Hong" last="Gao">Hong Gao</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name><affiliation wicri:level="1"><nlm:aff id="aff2">The Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Dong, Xiaoping" sort="Dong, Xiaoping" uniqKey="Dong X" first="Xiaoping" last="Dong">Xiaoping Dong</name><affiliation wicri:level="1"><nlm:aff id="aff3">Institute for Virology, Chinese Center for Disease Control and Prevention, Beijing 100052, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Virology, Chinese Center for Disease Control and Prevention, Beijing 100052</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wei, Jianguo" sort="Wei, Jianguo" uniqKey="Wei J" first="Jianguo" last="Wei">Jianguo Wei</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Dong, Guanmu" sort="Dong, Guanmu" uniqKey="Dong G" first="Guanmu" last="Dong">Guanmu Dong</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for the Control of Pharmaceutical and Biological Products, Tiantan Xili 2, Beijing 100050, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for the Control of Pharmaceutical and Biological Products, Tiantan Xili 2, Beijing 100050</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yin, Weidong" sort="Yin, Weidong" uniqKey="Yin W" first="Weidong" last="Yin">Weidong Yin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Chlorocebus aethiops</term><term>Convalescence</term><term>Humans</term><term>Neutralization Tests</term><term>Reference Standards</term><term>SARS Virus (immunology)</term><term>SARS Virus (physiology)</term><term>Serologic Tests (standards)</term><term>Severe Acute Respiratory Syndrome (blood)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Cellules Vero</term><term>Convalescence</term><term>Humains</term><term>Normes de référence</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (sang)</term><term>Tests de neutralisation</term><term>Tests sérologiques (normes)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Tests sérologiques</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Serologic Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Convalescence</term><term>Humans</term><term>Neutralization Tests</term><term>Reference Standards</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Convalescence</term><term>Humains</term><term>Normes de référence</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="May, J C" uniqKey="May J">J.C. May</name></author><author><name sortKey="Grim, E" uniqKey="Grim E">E. Grim</name></author><author><name sortKey="Wheeler, R M" uniqKey="Wheeler R">R.M. Wheeler</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Jiansan" sort="Zhang, Jiansan" uniqKey="Zhang J" first="Jiansan" last="Zhang">Jiansan Zhang</name></noRegion><name sortKey="Chen, Jiangting" sort="Chen, Jiangting" uniqKey="Chen J" first="Jiangting" last="Chen">Jiangting Chen</name><name sortKey="Dong, Guanmu" sort="Dong, Guanmu" uniqKey="Dong G" first="Guanmu" last="Dong">Guanmu Dong</name><name sortKey="Dong, Xiaoping" sort="Dong, Xiaoping" uniqKey="Dong X" first="Xiaoping" last="Dong">Xiaoping Dong</name><name sortKey="Gao, Hong" sort="Gao, Hong" uniqKey="Gao H" first="Hong" last="Gao">Hong Gao</name><name sortKey="Gao, Qiang" sort="Gao, Qiang" uniqKey="Gao Q" first="Qiang" last="Gao">Qiang Gao</name><name sortKey="Gong, Xuejie" sort="Gong, Xuejie" uniqKey="Gong X" first="Xuejie" last="Gong">Xuejie Gong</name><name sortKey="Hu, Liangxiang" sort="Hu, Liangxiang" uniqKey="Hu L" first="Liangxiang" last="Hu">Liangxiang Hu</name><name sortKey="Huang, Jinfeng" sort="Huang, Jinfeng" uniqKey="Huang J" first="Jinfeng" last="Huang">Jinfeng Huang</name><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><name sortKey="Li, Shufen" sort="Li, Shufen" uniqKey="Li S" first="Shufen" last="Li">Shufen Li</name><name sortKey="Liu, Yufen" sort="Liu, Yufen" uniqKey="Liu Y" first="Yufen" last="Liu">Yufen Liu</name><name sortKey="Liu, Yuxuan" sort="Liu, Yuxuan" uniqKey="Liu Y" first="Yuxuan" last="Liu">Yuxuan Liu</name><name sortKey="Ning, Ye" sort="Ning, Ye" uniqKey="Ning Y" first="Ye" last="Ning">Ye Ning</name><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name><name sortKey="Song, Lifei" sort="Song, Lifei" uniqKey="Song L" first="Lifei" last="Song">Lifei Song</name><name sortKey="Wei, Jianguo" sort="Wei, Jianguo" uniqKey="Wei J" first="Jianguo" last="Wei">Jianguo Wei</name><name sortKey="Yin, Weidong" sort="Yin, Weidong" uniqKey="Yin W" first="Weidong" last="Yin">Weidong Yin</name><name sortKey="Zhang, Xiaomei" sort="Zhang, Xiaomei" uniqKey="Zhang X" first="Xiaomei" last="Zhang">Xiaomei Zhang</name><name sortKey="Zhang, Zhenshan" sort="Zhang, Zhenshan" uniqKey="Zhang Z" first="Zhenshan" last="Zhang">Zhenshan Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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